Standard Svensk standard · SS-EN ISO 15216-1:2017

Mikrobiologi i livsmedelskedjan - Horisontell metod för bestämning av hepatit A virus och norovirus i livsmedel med realtids RT-PCR- Del 1: Metod för kvantifiering (ISO 15216-1:2017)

Status: Gällande

Omfattning
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

Ämnesområden

Livsmedelsmikrobiologi


Produktinformation

Språk: Engelska

Framtagen av: Livsmedelsanalyser, SIS/TK 435/AG 05

Internationell titel: Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)

Artikelnummer: STD-8025881

Utgåva: 1

Fastställd: 2017-04-06

Antal sidor: 64