This International Standard specifies a method for the determination of the contents of free a-, ß-, ?-, and d-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this International Standard.NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.