This document specifies requirements and recommendations to laboratories that perform extraction of polymerase chain reaction (PCR) quality deoxyribonucleic acid (DNA) from cottonseed, cotton leaf and raw material derived therefrom, that is sufficient for the purpose of PCR analysis.


This document is applicable to:


a)       identifying cotton raw material from which PCR quality DNA can be extracted;


b)       specifying a method for effective DNA extraction from cotton and cotton-derived raw materials;


c)        specifying the cotton-specific marker(s) to be used as controls for PCR amplification of DNA.


A PCR result obtained from analysis of cottonseed, cotton leaf and to some extant raw materials derived therefrom can only indicate that it is not derived from pure genetically modified organism (GMO)-derived cotton. Admixtures of GMO-derived cotton cannot be detected for cotton fibre a...