Standard Swedish standard · SS-EN 12823-1:2014

Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 1: Measurement of all-E-retinol and 13-Z-retinol

Status: Valid

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Standard Swedish standard · SS-EN 12823-1:2014

Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 1: Measurement of all-E-retinol and 13-Z-retinol
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Scope
This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and ß-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol.
The extract obtained after saponification in this method can be used for the determination of ß-carotene, as described in EN 12823 2:2000 Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of ß-carotene. In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerisation and oxidation of ß-carotene.

Subjects

Processes in the food industry (67.020) General methods of tests and analysis for food products (67.050)


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Standard Swedish standard · SS-EN 12823-1:2014

Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 1: Measurement of all-E-retinol and 13-Z-retinol
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Price: 755 SEK
standard ikon pdf

PDF

Price: 755 SEK
standard ikon

Paper

Price: 1 208 SEK
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Product information

Language: English

Written by: SIS - Miljö och energi

International title:

Article no: STD-102029

Edition: 2

Approved: 6/1/2014

No of pages: 28

Replaces: SS-EN 12823-1