Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains
This European Standard defines general rules for the enumeration of probiotic yeasts in feed samples (additives, premixtures and feeding stuffs) that contain yeast as a single microorganism component or in a mixture with other microorganisms. The standard is not applicable to mineral feeds which are defined as complementary feedingstuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) .
There are different categories of feed samples:
a) Additives which contain about 109 CFU/g to 1010 CFU/g (CFU = colony forming units):
b) Premixtures which contain about 108 CFU/g,
c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feedingstuffs, and milk replacers.
The detection limit is as defined in EN ISO 7218.