This document, part of EN 13130, specifies two methods as follows.
Method A is an analytical procedure for the determination of the monomers 1,2-DHB, 1,3-DHB and 1,4-DHB in the food simulants water, 3 % w/v aqueous acetic acid, 15 % v/v aqueous ethanol and olive oil. The levels of monomer determined are expressed in milligrams of monomer per kilogram of food simulant. The method is appropriate for the quantitative determination of 1,2-DHB and 1,3-DHB at a minimum level of 0,15 mg/kg in food simulants and, of 1,4-DHB at a minimum level of 0,10 mg/kg in food simulants.
Method B is an analytical procedure for the determination of DHBphen and DHBiph in the food simulants water, 3 % w/v aqueous acetic acid, 15 % v/v aqueous ethanol and olive oil. The method is appropriate for the quantitative determination of DHBphen or DHBiph at minimum levels of 0,2 mg/kg and 0,25 mg/kg, respectively, in food simulants.
NOTE The methods should also be applicable to other aqueous food simulants as well as to the other fatty food simulants e.g. sunflower oil, corn oil or a mixture of synthetic triglycerides.