1.1 This test method covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods.1.2 The detection limit is one colony forming unit (CFU)/mL metalworking fluid.1.3 This test method involves culture of organisms classified as Level 2 pathogens, and should be